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Unsourced material may be challenged and removed. Please help improve this article by adding citations to reliable sources. Length measurement mathematical morphology stereology tube formation validation.This article needs additional citations for verification. Using this new automated procedure, we are able to treat 540 images, or three complete assays per hour. The resulting image analysis software is currently running on a dedicated server, with comparable accuracy to manual measurements. More than 800 images from 12 experiments were analyzed automatically and manually, and their results were compared to improve and validate the proposed image analysis procedure. We propose a series of image analysis procedures developed using a freely available image analysis software library. In this article, we propose an automated procedure to evaluate the pseudo-tubule network lengths. Moreover, the screening is operator dependent. This standard measurement is usually carried out manually by trained operators but requires time, attention, and dedication to achieve a reasonable degree of accuracy. This assay is based on quantification of the stimulatory and inhibitory effects of various agents, which is estimated through the measurement of the pseudo-tubules network length. One of the most informative assays is the endothelial cells capillary tube formation assay performed on a biological matrix. In vitro angiogenesis assays provide a platform for evaluating the effects of pro- or antiangiogenic compounds. It is a fundamental component of a number of normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth, and metastasis). Angiogenesis is the biological process of generating new capillary blood vessels.
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